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ARS Home » Southeast Area » Auburn, Alabama » Aquatic Animal Health Research » Research » Publications at this Location » Publication #251243
Title: Identification and expresion profile of multiple genes in channel catfish fry ten minutes after modified live Flavobacterium columnare vaccination

Author
item Wei Pridgeon, Yuping
item Klesius, Phillip

Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/11/2010
Publication Date: 10/5/2010
Citation: Wei Pridgeon, Y., Klesius, P.H. 2010. Identification and expresion profile of multiple genes in channel catfish fry ten minutes after modified live Flavobacterium columnare vaccination. Veterinary Immunology and Immunopathology. 138:25-33.

Interpretive Summary: Using subtractive hybridization technique, 32 genes were isolated from a channel catfish fry subtractive library ten minutes post vaccination with a modified live vaccine of Flavobacterium columnare. The transcription levels of the 32 genes in response to vaccination were then evaluated by quantitative PCR (QPCR). Of the 32 genes, 28 were induced higher in at least one vaccinated fish. Of the 28 induced genes, 12 were consistently induced at least 2 fold higher in vaccinated fish compared to unvaccinated control fish. Of the 12 highly induced genes, three were consistently induced more than 3 fold. The three genes were: triglyceride lipase, PIKK family atypical protein kinase, and CCR4-NOT transcription complex subunit 1. Other genes that were induced by vaccination also included CD59, polymerase (RNA) I polypeptide C, pyrophosphatase (inorganic) 1, mannose-P-dolichol utilization defect 1, Nascent polypeptide-associated complex subunit alpha, hemoglobin-beta, fetuin-B, glyoxalase domain containing 4, and putative histone H3. The 28 induced genes have putative functions in the following five major categories: (1) immune response (46%); (2) signal transduction (21%); (3) transcriptional regulation (11%); (4) cell maintenance (11%); and (5) unknown (11%).

Technical Abstract: Using PCR-select subtractive cDNA hybridization technique, 32 expressed sequence tags (ESTs) were isolated from 96 clones of a channel catfish (Ictalurus punctatus) fry subtractive library ten minutes post vaccination with a modified live Flavobacterum columnare vaccine. The transcription levels of the 32 ESTs in response to F. columnare vaccination were then evaluated by quantitative PCR (QPCR). Of the 32 ESTs, 28 were upregulated in at least one vaccinated fish. Of the 28 upregulated ESTs, 12 were consistently induced at least 2 fold higher in vaccinated fish compared to unvaccinated control fish. Of the 12 upregulated genes, three (triglyceride lipase, PIKK family atypical protein kinase, and CCR4-NOT transcription complex subunit 1) were consistently upregulated greater than 3 fold. The 12 consistently upregulated genes also included CD59, polymerase (RNA) I polypeptide C, pyrophosphatase (inorganic) 1, mannose-P-dolichol utilization defect 1, Nascent polypeptide-associated complex subunit alpha, hemoglobin-beta, fetuin-B, glyoxalase domain containing 4, and putative histone H3. The 28 upregulated ESTs represent genes with putative functions in the following five major categories: (1) immune response (46%); (2) signal transduction (21%); (3) transcriptional regulation (11%); (4) cell maintenance (11%); and (5) unknown (11%).