Isolation and characterization of microsatellite loci from the soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae)

Authors: KIM, HYOJOONG - Seoul National University; KIM, MIN-YOUNG - Seoul National University; KIM, KYUNG SEOK - Seoul National University; LEE, HANG - Seoul National University; Hoelmer, Kim; LEE, SEUNGHWAN - Seoul National University

Submitted to: Molecular Ecology Resources
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/18/2010
Publication Date: 7/27/2010
Citation: Kim, H., Kim, M., Kim, K., Lee, H., Hoelmer, K.A., Lee, S. 2010. Isolation and characterization of microsatellite loci from the soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae). Molecular Ecology Resources. 10:1098-1105.

Interpretive Summary: A decade ago the Asian soybean aphid was accidentally introduced into North America and it has since become the most serious pest of soybeans in the “soybean belt” of the north-central USA. In order to develop effective management methods for the soybean aphid on a regional scale, it is important to better understand the genetics of aphid populations. To accomplish this, genetic microsatellite markers were identified from aphid samples collected at various locations in Asia and the USA. These markers will facilitate further research on the genetics of soybean aphid populations.

Technical Abstract: Ten novel microsatellite loci were isolated and characterized from the soybean aphid, APHIS GLYCINES, a serious invasive pest of soybeans in North America. The isolated loci were polymorphic, with two to 19 alleles in 48 wild individuals collected from Korea, Japan, and the United States. All loci were successfully cross-amplified in the closely related cotton-melon aphid, APHIS GOSSYPII. The analyses revealed that 32 individuals had different multilocus genotypes, showing a great potential variability within and between populations, and facilitating further population genetic studies of the soybean aphid.