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Title: Association of a single nucleotide polymorphism (SNP) of calpain 1 (CAPN1) gene with meat tenderness of yak.

Author
item WU, X - Gansu Agricultural Uiversity
item YANG, L - Gansu Agricultural Uiversity
item WAN, H - Gansu Agricultural Uiversity
item ZHANG, L - Gansu Agricultural Uiversity
item WANG, J - Gansu Agricultural Uiversity
item Brown, Michael
item WU, J - Gansu Agricultural Uiversity

Submitted to: Journal of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: 4/18/2010
Publication Date: 8/4/2010
Citation: Wu, X.J., Yang, L., Wan, H.L., Zhang, L.P., Wang, J.H., Brown, M.A., Wu, J.P. 2010. Association of a single nucleotide polymorphism (SNP) of calpain 1 (CAPN1) gene with meat tenderness of yak [abstract]. American Society of Animal Science Annual Meeting, July 11-15, 2010, Denver, CO. Available on-line: http://adsa.asas.org/meetings/2010/toc.asp

Interpretive Summary: Abstract only.

Technical Abstract: The association of a single nucleotide polymorphism (SNP) of calpain 1 (CAPN1) gene with shear force of 2.54 cm steaks from M. longissimus dorsi from Gannan yaks (Bos grunniens, n=181) was studied. Yaks were harvested at 2, 3, and 4 yr of age (n=51, 59, and 71, respectively), and samples of each yak carcass were aged at 4°C for 0, 1, 3, 7, 14 or 21d. Shear force for each sample was determined at each of the six postmortem aging-day using Warner-Bratzler shear force methodology. Fragments of the yak CAPN1 gene including exon 9 and intron 9 were ampli'ed and subjected to Single Strand Conformation Polymorphism analysis. A SNP was found within intron 9 of the CAPN1 gene G5837A, and there were two genotypes of GG and GA identified, respectively. The genotypic frequency of GG was 82.87% compared to 17.13% for GA. Statistical analysis was done using mixed model procedures. Interaction of SNP x age at harvest x aging days was demonstrated (P < 0.10) for shear force. In 2-yr-old yak, genotype GG had lesser shear force than that of GA at the aging times of 0, 3, and 7 days (0.78, 0.92, and 0.65 kg, respectively; P < 0.05); however, in 3-yr- and 4-yr-old yak, genotype GG had lesser shear force than GA at aging time of 14 days (0.71 and 0.58 kg, respectively; P < 0.05). Genotype had little effect (P > 0.05) on shear force for 3- and 4-yr-old yaks averaged over aging time. In 2-yr-old yak, genotype GG had 0.57 kg lesser shear force than yaks with genotype GA (P < 0.05) averaged over aging time. Therefore, it is evident that the CAPN1 SNP influenced (P < 0.01) shear force of M. longissimus dorsi depending on age of yak and aging time of the meat. Further study should focus on the ontogeny of CAPN1 gene mRNA expression.