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United States Department of Agriculture

Agricultural Research Service

Research Project: DEVELOPMENT AND ASSESSMENT OF A SYSTEM TO PRODUCE GRASS-FED BEEF FOR THE SOUTHERN GREAT PLAINS Title: Association of a single nucleotide polymorphism (SNP) of calpain 1 (CAPN1) gene with meat tenderness of yak.

Authors
item Wu, X -
item Yang, L -
item Wan, H -
item Zhang, L -
item Wang, J -
item Brown, Michael
item Wu, J -

Submitted to: Journal of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: April 18, 2010
Publication Date: August 4, 2010
Citation: Wu, X.J., Yang, L., Wan, H.L., Zhang, L.P., Wang, J.H., Brown, M.A., Wu, J.P. 2010. Association of a single nucleotide polymorphism (SNP) of calpain 1 (CAPN1) gene with meat tenderness of yak [abstract]. American Society of Animal Science Annual Meeting, July 11-15, 2010, Denver, CO. Available on-line: http://adsa.asas.org/meetings/2010/toc.asp

Interpretive Summary: Abstract only.

Technical Abstract: The association of a single nucleotide polymorphism (SNP) of calpain 1 (CAPN1) gene with shear force of 2.54 cm steaks from M. longissimus dorsi from Gannan yaks (Bos grunniens, n=181) was studied. Yaks were harvested at 2, 3, and 4 yr of age (n=51, 59, and 71, respectively), and samples of each yak carcass were aged at 4°C for 0, 1, 3, 7, 14 or 21d. Shear force for each sample was determined at each of the six postmortem aging-day using Warner-Bratzler shear force methodology. Fragments of the yak CAPN1 gene including exon 9 and intron 9 were ampli'ed and subjected to Single Strand Conformation Polymorphism analysis. A SNP was found within intron 9 of the CAPN1 gene G5837A, and there were two genotypes of GG and GA identified, respectively. The genotypic frequency of GG was 82.87% compared to 17.13% for GA. Statistical analysis was done using mixed model procedures. Interaction of SNP x age at harvest x aging days was demonstrated (P < 0.10) for shear force. In 2-yr-old yak, genotype GG had lesser shear force than that of GA at the aging times of 0, 3, and 7 days (0.78, 0.92, and 0.65 kg, respectively; P < 0.05); however, in 3-yr- and 4-yr-old yak, genotype GG had lesser shear force than GA at aging time of 14 days (0.71 and 0.58 kg, respectively; P < 0.05). Genotype had little effect (P > 0.05) on shear force for 3- and 4-yr-old yaks averaged over aging time. In 2-yr-old yak, genotype GG had 0.57 kg lesser shear force than yaks with genotype GA (P < 0.05) averaged over aging time. Therefore, it is evident that the CAPN1 SNP influenced (P < 0.01) shear force of M. longissimus dorsi depending on age of yak and aging time of the meat. Further study should focus on the ontogeny of CAPN1 gene mRNA expression.

Last Modified: 11/1/2014
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