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United States Department of Agriculture

Agricultural Research Service

Research Project: DEVELOPMENT AND ASSESSMENT OF A SYSTEM TO PRODUCE GRASS-FED BEEF FOR THE SOUTHERN GREAT PLAINS

Location: Forage and Livestock Production Unit

Title: The effects of single nucleotide polymorphisms (SNPs) of calpastatin (CAST) gene on meat tenderness of yak.

Authors
item Wang, J -
item Wu, J -
item Wan, H -
item Yang, L -
item Wu, X -
item Brown, Michael
item Zhang, L -

Submitted to: Journal of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: April 15, 2010
Publication Date: August 4, 2010
Citation: Wang, J.H., Wu, J.P., Wan, H.L., Yang, L., Wu, X.J., Brown, M.A., Zhang, L.P. 2010. The effects of single nucleotide polymorphisms (SNPs) of calpastatin (CAST) gene on meat tenderness of yak [abstract]. American Society of Animal Science Annual Meeting, July 11-15, 2010, Denver, CO. Available on-line: http://adsa.asas.org/meetings/2010/toc.asp

Interpretive Summary: Abstract only.

Technical Abstract: The association of single nucleotide polymorphisms (SNPs) of calpastatin (CAST) gene with shear force of 2.54 cm steaks from M. longissimus dorsi from Gannan yaks (Bos grunniens, n=181) was studied. Yaks were harvested at 2, 3, and 4 yr of age (n=51, 59, and 71, respectively), and samples of each yak carcass were aged at 4°C for 0, 1, 3, 7, 14 or 21d. Shear force for each sample was determined at each of the six postmortem aging-day using Warner-Bratzler shear force methodology. Fragments of the yak CAST gene including exon 3 were amplified and subjected to Single Strand Conformation Polymorphism analysis. Two linkage SNPs were found within exon 3 of the CAST gene: G40455A and G40463A, and there were two genotypes of GG/AA and GA/GA demonstrated respectively. The genotypic frequency of GG/AA was 72.93% compared to 27.07% for GA/GA. Statistical analysis was done using mixed model procedures. Interaction of genotype x age at harvest x aging days was demonstrated (P < 0.05) for shear force. In 2-yr-old yak, GG/AA had lesser shear force than GA/GA at the aging times of 1 and 7 days (1.15 and 0.57 kg, respectively; P < 0.05); 4-yr-old yak GG/AA had lesser shear force than GA/GA at aging times of 7 and 14 days (0.64 and 0.49 kg, respectively; P < 0.05). Genotype had little effect (P > 0.05) on shear force in 2-yr- and 3-yr-old yak averaged over aging time. In 4-yr-old yak, GG/AA had 0.40 kg lesser shear force than GA/GA (P < 0.05) averaged over aging time. Therefore, it was concluded that the CAST SNPs affect (P < 0.10) shear force of M. longissimus dorsi depending on age of yak and aging time of the meat. Further study should focus on the ontogeny of CAST gene mRNA expression.

Last Modified: 8/1/2014