Submitted to: Electronic Journal of Biotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: August 25, 2010
Publication Date: November 15, 2010
Repository URL: http://www.scielo.cl/pdf/ejb/v13n6/a05.pdf
Citation: Yuan, M., Gong, L., Meng, R., Li, S., Dang, P.M., Guo, B., He, G. 2010. Development of trinucleotide (GGC)n SSR markers in peanut (Arachis hypogaea L.). Electronic Journal of Biotechnology. 13:6. DOI: 10.2225/vol13-issue6-fulltext-6. Interpretive Summary: Cultivated peanut is one of the most important oilseed crops due to its valuable source of vegetable oil and protein. However, improvement of peanut production by molecular tools has been difficult because of its narrow genetic base which originated from a single and recent polyploidization event. Simple sequence repeat (SSR) markers are very informative compared to other molecular marker systems. Up to date, thousands of SSRs available in peanut, but only about 10% of them are polymorphic. The effort has been made to obtain all SSR markers from both genomic and EST sources. The (GGC)n SSRs identified in this study represents new type of SSR markers in peanut with a related information on their abundance and informativeness. Despite the low number of polymorphic markers in cultivated peanut, two SSR markers were mapped to an existing linkage map of cultivated peanut. A high level of transferability of (GGC)n SSRs to wild species facilitates the construction of genetic linkage maps in wild species, and accelerates the introgression of wild useful genes into cultivated peanut.
Technical Abstract: Cultivated peanut (Arachis hypogaea L.) is an agronomical and economical important oilseed crop. It is native to South America, but it is grown extensively in the semi-arid tropics of Asia, Africa, and Latin America. Because its genetic base is narrow, a pressed effort has been made to develop SSR markers to provide useful genetic and genomic tools for peanut research community. We have constructed an SSR-enriched library to isolate trinucleotide (GGC)n SSRs in peanut. A total of 143 unique sequences containing (GGC)n repeats were identified. One hundred thirty eight primer pairs were successfully designed at the flanking region of SSRs. A suitable polymerase was chosen to amplify these GC-rich sequences. Although a low level of polymorphism was shown by these new developed SSRs, a high level of transferability to wild species would facilitate the introgression of useful genes from wild species to cultivated peanut.