|Han, Sung Nim -|
|Pang, Eunice -|
|Zingg, Jean-Marc -|
|Meydani, Simin -|
|Meydani, Mohsen -|
|Azzi, Angelo -|
Submitted to: Archives Of Biochemistry and Biophysics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 21, 2009
Publication Date: February 1, 2010
Citation: Han, S., Pang, E., Zingg, J., Meydani, S.N., Meydani, M., Azzi, A. 2010. Differential effects of natural and synthetic vitamin E on gene transcription in murine T lymphocytes. Archives Of Biochemistry and Biophysics. 495:49-55. Interpretive Summary: The in vivo effects of natural and synthetic vitamin E have been studied in mice by supplementing their diet with the two forms of the vitamin. The influence of the two types of vitamin E on the gene expression of activated T lymphocytes was studied. The results show that the two vitamin E forms have substantially different effects in terms of gene expression modulation. We found that some genes are uniquely expressed by natural vitamin E. This information could be used in guiding health professionals regarding biomarkers of in vivo supplementation with synthetic and natural forms of vitamin E.
Technical Abstract: Mice were supplemented with low and high doses of natural and synthetic vitamin E, T cells from the spleen isolated and stimulated with plate-bound anti-CD3 and soluble anti-CD28, and gene expression changes assessed by gene array experiments. The data obtained indicate significant qualitative and quantitative differences between the two vitamin forms in regulating gene expression induced by T cell stimulation. Marker genes have been found whose expression can be considered significant in establishing the level of, and response to vitamin E for both natural and synthetic vitamin E supplementation; unique markers for synthetic vitamin E supplementation and unique markers for natural vitamin E supplementation have been identified.