ARS | Publication request: Characterization of the Recombinant Inbred Line Population Derived from the Cross of Nipponbare/9311

Submitted to: Rice Technical Working Group Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: January 15, 2010
Publication Date: February 22, 2010
Citation: Liu, G., Jia, Y., Jia, M.H., Venu, R., Wang, G., Meyers, B., McClung, A.M. 2010. Characterization of the Recombinant Inbred Line Population Derived from the Cross of Nipponbare/9311. In: Proceedings of the 33rd Rice Technical Working Group Meeting, Feb. 22-24, 2010, Biloxi, MS. CDROM

Technical Abstract: As a part of the project entitled “Understanding the rice epigenome: From genes to genomes” funded by the National Science Foundation, a mapping population of 480 F6-8 recombinant inbred lines (RILs) derived from a cross of Nipponbare with 9311 (Nip/9311) was developed. Phenotyping important agronomic traits and genotyping of the population was conducted at USDA-ARS Dale Bumpers National Rice Research Center, Stuttgart, AR and Rice Research Unit, Beaumont, TX in 2009. To date, phenotypic transgressive variation among the Nip/9311 RILs was observed and two hundred and seventy Nip/9311 RILs were genotyped using 200 simple sequence repeat (SSR) markers to establish a linkage map. The goal of this project will lead to the identification of four best-performing lines that outperform both parental lines for yield, yet retain a significant combination of both parental genomes based on genotypic data. Comparisons of epigenomic profiles between parental lines, F1 hybrids and selected RILs should provide information on the inheritance of DNA methylation, histone modification and imprinting over multiple generations, and may help elucidate their roles in hybrid vigor and heterosis. Ultimately, a broader set of these lines will be analyzed to gain a better understanding of molecular mechanisms of heterosis.