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United States Department of Agriculture

Agricultural Research Service

Research Project: SYSTEMATICS, GENETIC DIVERSITY ASSESSMENT, AND ACQUISITION OF POTATOES, CARROTS, AND THEIR RELATED WILD RELATIVES

Location: Vegetable Crops Research Unit

Title: Evolution of Chromosome 6 of Solanum Species Revealed by Comparative Fluorescence in Situ Hybridization Mapping

Authors
item Luo, Qunfeng -
item Iovene, Marina -
item Spooner, David
item Buell, C -
item Jiang, Jiming -

Submitted to: Chromosoma
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 19, 2010
Publication Date: May 2, 2010
Citation: Luo, Q., Iovene, M., Spooner, D.M., Buell, C.R., Jiang, J. 2010. Evolution of Chromosome 6 of Solanum Species Revealed by Comparative Fluorescence in Situ Hybridization Mapping. Chromosoma. 119:435-442.

Interpretive Summary: Potatoes, tomatoes, and eggplants are economically significant vegetable crops with wild species relatives of use in their crop breeding. All three of these crops are related and grouped in the genus Solanum. Recently developed methods are allowing us to use the power DNA to understand how organisms are related to each other, and to use this information in a variety of useful ways, such as plant breeding. This study, called fluorescent in situ hybridization (FISH), builds upon earlier studies using DNA to infer the relationships of species in the genus Solanum. It uses large pieces of DNA (called BAC clones) to show the comparative arrangement of the genetic strands in these plants called chromosomes. Some of the plants we analyzed have big pieces of their chromosomes flipped around (inverted) relative to other plants. We can use these inversions, in concert with prior studies, to infer how these plants are related to each-other. We also can use these results to infer how useful these plants are to each other in plant breeding programs.

Technical Abstract: Comparative genome mapping is an important tool in evolutionary research. Here we demonstrate a comparative fluorescent in situ hybridization (FISH) mapping strategy. A set of 13 bacterial artificial chromosome (BAC) clones derived from potato chromosome 6 was used for FISH mapping in seven different Solanum species. FISH mapping revealed the ancestral karyotype of this chromosome and as well as one paracentric inversion and one pericentric inversion in specific lineages. We demonstrate that comparative FISH mapping is an efficient and powerful methodology to study chromosomal evolution among related plant species.

Last Modified: 7/28/2014
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