Location: Plant Genetics Research
Title: DNA Sequence Characterization of a Gene-rich Region in Rye Authors
Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 22, 2009
Publication Date: January 15, 2010
Repository URL: http://www.intl-pag.org/18/abstracts/W45_PAGXVIII_345.html
Citation: Gustafson, J.P., Ross, K. 2010. DNA Sequence Characterization of a Gene-rich Region in Rye. Plant and Animal Genome Conference Proceedings. Technical Abstract: Aluminum toxicity, affecting approximately half of the world’s arable land, severely hinders the ability of crop plants to utilize moisture and nutrients by restricting root growth and function. Among the cultivated cereals, rye is the most Al-tolerant of the cereals and represents an important potential source of genes for the improvement of Al-tolerance in wheat. High-resolution mapping at the Alt4 Al-tolerance locus previously identified a cluster of genes including at least four ALMT1 malate transporter genes. Therefore, we decided to sequence a rye BAC clone containing genes controlling aluminum tolerance. The sequence characterization of a 135 kb rye BAC clone established the presence of a gene-rich region containing four genes including an ALMT1, a zinc-finger transcription factor, a cytochrome P450, and OsB28 (putative pentatricopeptide). The BAC sequence clearly showed, that at least for this region of the rye genome, a 100% linear relationship existed between rye and rice. In addition, sequencing indicated the evolutionary factors causing the vast expansion in size of the rye genome compared to the rice genome.