Technical Abstract: Honey bees provide excellent opportunities for studying development, behavior, and defenses against natural parasites and pathogens. They are also a critical component of modern agriculture, through their leading role in pollinating the world’s crops. Research and diagnostic methods for honey bees require efficient sampling methods for all colony members, often involving a large number of individual bees. This need for greater sampling must be balanced by the expenses involved with collecting and screening numerous colony members. In the case of whole-colony surveys for pathogens, mass extractions from adult workers require scaled up amounts of expensive reagents and, as a result, most such surveys have been limited to a small subset of colony members. Mass removal of viable embryos can be used for diagnostics, as a screening tool for genetic traits of interest and, in principle, an efficient first step in programs for in vitro rearing of honey bee workers, queens, and drones. Here we describe a simple method for collecting thousands of honey bee embryos and show that these embryos have higher hatching rates than do embryos collected by more laborious methods.