ARS | Publication request: Development of genetic resources for association mapping in pea

Submitted to: Meeting Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 2009
Publication Date: July 16, 2009
Citation: Coyne, C.J., Diesso, D., Vandemark, G.J., Timmerman-Vaughan, G., Brown, A.F. 2009. Development of genetic resources for association mapping in pea. Meeting Proceedings. Model Legume Congress, Asilomar Conference Grounds, July 12-16, 2009.

Technical Abstract: A previous study of the allelic diversity of the USDA pea core collection resequenced ten loci of 32 diverse pea germplasm accessions from which SNP assays were designed for Agpl1, AspS2, Cwi1, DRR206c, PPlike, PsAS2, SOD9, Thaumatin1, PsLD, and Viola2 (Aubert et al. 2006; Gilpin et al 1997; Hecht et al 2005). As commonly found, two genotypes per SNP were found in these ten pea gene sequences. Allele discrimination between the two alleles of each single nucleotide polymorphism was determined using a multiplex TaqMan assay. The results of genotyping 217 of 384 pea core accessions with two SNP assays found high levels of homozygosity as expected in this single seed descent population. This baseline information on the pea core may contribute to association mapping (linkage disequilibrium) studies in Pisum. The data will be available on the public web site maintained by the USDA-Agricultural Research Service for plant genetic resources (www. http://www.ars-grin.gov/npgs/searchgrin.html) and seed may be requested from the first author at coynec@wsu.edu.