Technical Abstract: Hirsutella minnesotensis is a fungal endoparasite of nematodes juvenile and parasitizes soybean cyst nematodes (SCN) with high frequency. In this study, the effects of two H. minnesotensis isolates on population and distribution of SCN and growth of soybean were evaluated. Experiments were conducted using 0.4 g of fresh mycelium/300 cm3 autoclaved soil (per pot) against soybean cyst nematode (9,000 eggs /300 cm3 soil) in the greenhouse. Thirty-five days after inoculation on soybean, H. minnesotensis isolates 1-10 and HLJ07-21-3 increased soybean fresh weights by 30.98% and 27.66%, respectively. Inoculation with H. minnesotensis also significantly (P = 0.05) reduced the number of nematode eggs and the number of females on the root and cysts in the soil. Use of the isolates significantly (P < 0.05) decreased the densities of total eggs in the pot by 63.7% and 46.7% when compared with no H. minnesotensis treatment. The total number of nematodes (sum of eggs and J2) were reduced by 43.6% and 35.8% as compared with no H. minnesotensis. Isolate1-10 more efficiently reduced the nematode population than isolate HLJ07-21-3.