MANAGEMENT AND GENETIC CHARACTERIZATION OF AGRICULTURAL AND BIOTECHNOLOGICAL MICROBIAL RESOURCES
Location: Bacterial Foodborne Pathogens & Mycology Research Unit
Title: Emendation of the Genus Actinokineospora Hasegawa 1988 and Transfer of Amycolatopsis fastidiosa Henssen et al. 1987 as Actinokineospora fastidiosa comb. nov
Submitted to: International Journal of Systematic and Evolutionary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 27, 2009
Publication Date: June 1, 2010
Citation: Labeda, D.P., Price, N.P., Tan, G.A., Goodfellow, M., Klenk, H. 2010. Emendation of the Genus Actinokineospora Hasegawa 1988 and Transfer of Amycolatopsis fastidiosa Henssen et al. 1987 as Actinokineospora fastidiosa comb. nov. International Journal of Systematic and Evolutionary Microbiology. 60(6):1444-1449.
Interpretive Summary: Assessment of evolutionary relationships of microoganisms at the species, genus, or higher taxonomic levels through gene sequence-based molecular analyses has shown problems with existing classification of microbial species that were based on incomplete morphological or chemical composition data. The microorganism that produces a complex of macrobicyclic peptide antibiotics was originally described as a species of the genus Pseudonocardia in the U.S. patent that issued in 1977 and subsequently identified as the new species Amycolatopsis fastidiosa in 1987 based on new observations of the morphology and chemical composition of the microorganism. A molecular systematic analysis of the 16S ribosomal RNA gene sequences of all members of the bacterial family Pseudonocardineae, to which this strain belongs, revealed that it is not related to the genus Amycolatopsis, but is much more similar to the genus Actinokineospora, whose species exhibit similar biochemical composition. Although this species does not produce motile spores like the rest of the species in Actinokineospora, it is genetically and biochemically very similar to them and is now described as a species in this genus. The detailed morphological, physiological, and genetic information provided in the description of this species, particularly that species can exist that do not produce motile spores, should be useful to researchers in biotechnology in the search for additional strains of Actinokineospora to exploit as a source of new antibiotics and other useful compounds.
The species Amycolatopsis fastidiosa was proposed by Henssen et al. (1987), based on morphological and chemotaxonomic observations, for a strain originally described as 'Pseudonocardia fastidiosa' by Celmer et al. in a US Patent issued in 1977. In the course of a phylogenetic study of the valid taxa within the suborder Pseudonocardineae based on 16S rRNA gene sequences it became apparent that this strain was misplaced in the genus Amycolatopsis. After careful evaluation of the phylogeny, morphology, chemotaxonomy, and physiology of this strain it was concluded that is a species of the genus Actinokineospora that is unable to produce motile spores. The description of the genus Actinokineospora is therefore emended to accommodate species that do not produce motile spores and it is proposed that this species be transferred to the genus Actinokineospora as Actinokineospora fastidiosa comb. nov. The type strain of the species is NRRL B-16697T = ATCC 31181T = DSM 43855T = JCM 3276T = NBRC 14105 T = VKM Ac-1419 T.