Technical Abstract: Using high performance liquid chromatography combined with ESi, APCI, and PBEI mass spectroscopy, novel terpenoid glycoconjugates were identified in oral secretions of several Anastrepha fly species; these findings suggest that non-volatile pheromone signals are used in their lek mating strategies. The terpenoid aglycons, which originate in salivary glands and traverse through interconnected ducts to the crop, are likely conjugated enzymatically as glucosidases and glucotransferases often function in an anabolic capacity. The communicative function(s) of the glucoconjugates is likely tied to the integrity of the O-glucosyl ester linkage. Enzymic, or abiotic, hydrolysis of the glucoconjugates in the oral secretions would prolong the emission of from lek sites. This is curious from an evolutionary perspective, because many fruits control the release of their volatile attractants from sugar-based matrices in this manner. Alternatively, pheromone activity of the glucoconjugates, or the enzymatically liberated aglycon, may be related to the tasting of oral secretion scent markings by conspecifics, which is a common behavior within Tephrtidea.