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United States Department of Agriculture

Agricultural Research Service

Research Project: SOYBEAN GENETIC MANAGEMENT AND UTILIZATION

Location: Soybean/maize Germplasm, Pathology, and Genetics Research

Title: Wide Hybridization in Soybean

Authors
item Signh, Ram -
item Nelson, Randall

Submitted to: Soybean Research World Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: May 10, 2009
Publication Date: August 9, 2009
Citation: Signh, R.J., Nelson, R.L. 2009. Wide Hybridization in Soybean [abstract]. In: [abstract]. In: Proceedings of the World Soybean Research Conference VIII,August 9-16, 2009, Beijing, China. 2009 CDROM.

Technical Abstract: The 26 wild perennial species of the subgenus Glycine Willd. that are indigenous to Australia are an untapped source of genetic diversity for soybean breeding. The objectives of this research are to develop an efficient methodology by which G. tomentella Hayata (2n=78) can be hybridized with the soybean (2n=40) and self-fertile 2n=40, 41, and 42 progeny produced. PI 441001 (G. tomentella) was crossed to the cultivar Dwight. F1 plants contained the expected 2n=59 and amphidiploid (2n=118) plants were produced by colchicine treatment. Dwight was used as a recurrent parent for producing BC1 (2n=79) to BC5 plants. BC2 plants contained 2n=55 to 59 chromosomes and BC3 plants contained 2n=41 to 50 chromosomes. Plants with 2n=41 (monosomic alien addition lines; MAALs) were usually self fertile whereas plants with greater than 42-chromosomes were male sterile and backcrossed to Dwight. At least one 2n=42 genetically and chromosomally stable line has been identified. The progenies of MAALs produce approximately 70% plants with 2n=40, 28% plants with 2n=41 and 2% plants with 2n=42 (disomic alien addition lines; DAALs). DAALs and diploid lines are being screened for resistant to pest and pathogens, and changes in seed composition.

Last Modified: 8/30/2014
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