Technical Abstract: Soybean rust, caused by Phakopsora pachyrhizi Syd. & P. Syd., is one of the most destructive diseases of soybean (Glycine max (L.) Merr.) worldwide. To identify sources of resistance to domestic soybean rust isolates, our strategy has been evaluate soybean lines that were previously identified as resistant to foreign isolates. In this study, two sets of plant introductions (PI) were evaluated using a bulk population of P. pachyrhizi urediniospores collected in Mississippi in 2006. The first set of PIs contained 10 lines previously identified as resistant in Paraguay and four PIs with known single genes for resistance to P. pachyrhizi. The second set had 17 lines that were selected based on information from Germplasm Resources Information Network (GRIN). Soybean cv. Williams 82 and Freedom were used as susceptible checks. Replicated experiments were conducted in growth chambers at the Stoneville Research Quarantine Facility from 2006 to 2008. Four soybean lines had red-brown resistant reactions to soybean rust. PI567102B was identified as one of the most resistant lines.