ARS | Publication request: Tagging and mapping Pse-1 gene for resistance to halo blight in common bean differential cultivar UI-3

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: October 1, 2008
Publication Date: January 1, 2009
Citation: Miklas, P.N., Fourie, D., Wagner, J., Larsen, R.C., Mienie, C. 2009. Tagging and mapping Pse-1 gene for resistance to halo blight in common bean differential cultivar UI-3. Crop Science. 49:41-48.

Interpretive Summary: Halo blight disease of common bean (dry and garden beans) is widespread in the U.S. causing significant production problems in the Great Lakes and Northern High Plains regions. Genetic resistance is an effective means for control of the disease. Our work characterizes the inheritance of a gene which conditions resistance to four different strains of the bacterial pathogen. We identified markers tightly linked with the major gene which will be used by breeders for marker-assisted selection of the gene. Deployment of this gene, facilitated by marker-assisted selection, will lead to development of new cultivars of dry and snap bean with enhanced resistance to halo blight disease.

Technical Abstract: Halo blight [caused by Pseudomonas syringae pv. phaseolicola (Psp)] is a serious seed-borne bacterial disease of common bean (Phaseolus vulgaris L.). A few R genes and QTL provide control to one or more races of the pathogen. To better understand monogenic resistance and improve breeding efficiency we sought to tag and map a gene (Pse-1) in host differential cultivar UI-3 (previously named Red Mexican UI-3) which provides resistance to races 1, 5, 7, and 9 of Psp. Co-segregation for resistance to races 1, 5, 7 and 9, in a recombinant inbred population, Canadian Wonder/UI-3 (CU), confirmed the effect of Pse-1 against multiple races of the pathogen. Bulked-segregant analysis in the CU population identified six RAPD markers tightly linked (0 to 3.3 cM) to Pse-1. Three of the RAPDs completely linked with Pse-1 in the CU population were converted to SCAR markers SH11.800, SR13.1150 and ST8.1350. The linked markers were used to integrate Pse-1 to linkage group B10 of the core map. Allelism tests (F2) confirmed relationships of Pse-1 and Pse-4 derived from UI-3 with R genes in the other host differential cultivars. A survey of advanced lines and cultivars revealed that the SCAR markers generated in this study will have utility for MAS of Pse-1 in germplasm from the Andean gene pool (e.g., kidney, calima) and from race Mesoamerican within the Middle American gene pool (black, carioca).