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Title: Analysis Of Proteins Differentially Accumulated During Potato Late Blight Resistance Mediated by the RB Resistance Gene

Author
item Halterman, Dennis
item LIU, ZHENYU - UNIV OF WISC, MADISON

Submitted to: Physiological and Molecular Plant Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/2/2009
Publication Date: 11/10/2009
Citation: Halterman, D.A., Liu, Z. 2009. Analysis Of Proteins Differentially Accumulated During Potato Late Blight Resistance Mediated by the RB Resistance Gene. Physiological and Molecular Plant Pathology. 74(2):151-160.

Interpretive Summary: The RB gene cloned from the wild diploid potato species Solanum bulbocastanum confers resistance against the late blight pathogen, Phytophthora infestans. We have used molecular techniques to determine what proteins are differentially regulated in resistant versus susceptible plants. We identified 12 proteins that were significantly different in concentration in the resistant or susceptible plants after inoculation with P. infestans. The sequence of proteins were identified using mass spectrometry and have a putative role in photosynthesis and stress responses.

Technical Abstract: The RB gene cloned from the wild diploid potato species Solanum bulbocastanum confers resistance against the late blight pathogen, Phytophthora infestans. Two-dimensional gel electrophoresis followed by mass spectrometry was used to examine for the first time the changes of the proteome pattern of potato leaves in response to inoculation with P. infestans. A nearly isogenic system comprised of resistant transgenic potato (Solanum tuberosum cv. Katadhin) carrying RB and the susceptible control S. tuberosum cv. Katadhin was utilized for this study. Comparative analysis of 2DE gel maps revealed the presence of a total of 12 protein spots showing a significant difference (' twofold) in relative abundance in the resistant and susceptible potato plants after inoculation with P. infestans. Using MALDI-TOF mass spectrometry and pairwise sequence comparison by searching against database, the identification of 5 out of 12 spots was determined. The identified proteins have putative roles in photosynthesis and stress responses.