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ARS Home » Research » Publications at this Location » Publication #226527

Title: Bacteriocin Inducer Peptides

Author
item Stern, Norman
item SVETOCH, EDWARD - ST DEPT RUSSIA
item ERUSLANOV, BORLS - ST DEPT RUSSIA
item PERELYGIN, VLADIMIR - ST DEPT RUSSIA
item LEVCHUK, VLADIMIR - ST DEPT RUSSIA

Submitted to: Invention Report
Publication Type: Germplasm Release
Publication Acceptance Date: 6/14/2007
Publication Date: 4/8/2008
Citation: Stern, N.J., Svetoch, E.S., Eruslanov, B.V., Perelygin, V.V., Levchuk, V.P. 2008. Bacteriocin Inducer Peptides. Invention Report. Patent #7,354,904.

Interpretive Summary: Poultry borne Campylobacter and Salmonella continue to cause human disease. Antibiotic resistance of these bacteria has encouraged development of alternative therapeutic treatments. We purified and characterized a variety of proteins produced by inhibitory bacteria, termed “bacteriocins”. Although relatively expensive, our bacteriocins have an impressive range of effectiveness against a wide variety of disease causing bacteria in chickens. We increased the production of these bacteriocins by identifying unique signal peptides with specified terminal amino acids. We added an inducer bacterium to the fermentation and employed dilute bacterial media to further enhance bacteriocin production. To derive economic benefit, the pharmaceutical and food industry may use these approaches to produce bacteriocins used to reduce the taxpayers risk for poultry borne disease.

Technical Abstract: Novel peptides produced by bacteriocin-producing bacteria stimulate the production of bacteriocins in vitro. The producer bacteria are cultured in the presence of a novel inducer bacteria and a peptide having a carboxy terminal sequence of VKGLT in order to achieve an increase in bacteriocin production.