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ARS Home » Midwest Area » Madison, Wisconsin » U.S. Dairy Forage Research Center » Research » Publications at this Location » Publication #220478
Title: HIGH-RESOLUTION SOLUTION-STATE NMR OF UNFRACTIONATED PLANT CELL WALLS: POTENTIAL FOR BIOMASS SELECTION AND PROCESS OPTIMIZATION

Author
item Ralph, John
item MATTIAS, HEDENSTROM - UMEA UNIV, UMEA, SWEDEN
item LU, FACHUANG - UNIV OF WISCONSIN
item KIM, HOON - UNIV OF WISCONSIN
item YELLE, DANIEL - US FOREST PRODUCTS LAB
item HAMMEL, KENNETH - US FOREST PRODUCTS LAB

Submitted to: Proceedings of Pittcon Meeting
Publication Type: Proceedings
Publication Acceptance Date: 10/15/2007
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Detailed structural studies on the plant cell wall have traditionally been difficult. NMR is one of the preeminent structural tools, but obtaining high-resolution solution-state spectra has typically required fractionation and isolation of components of interest. With new methods for dissolution of, admittedly, finely divided plant cell wall material, the wall can now be studied by NMR. Exploiting the dispersion of 2D (and even 3D) NMR allows strikingly detailed structural analysis of the wall components without the need for isolation and fractionation. Furthermore, the structural “fingerprint” of the cell wall produced by 2D HSQC experiments, for example, is potentially unmatched by any other spectroscopy. Therefore, such dissolution/NMR methods potentially lend themselves well to chemometrics methods, once the issues of dealing with multivariate analyses on 2D NMR data have been addressed. Potential applications of whole-cell-wall NMR spectroscopy and associated chemometrics methods include selection of ideal biomass material for bioconversion, delineation of degradation mechanisms (e.g. by white-rot fungi that preferentially degrade lignins), and optimization of biomass processing and conversion methods.