Author
YOSHIDA, YASUKAZU - NIAIST, JAPAN | |
LI, LEI - JM USDA HNRCA @ TUFTS | |
DUKER, J - TNEMC EYE CENTER | |
NIKI, ETSUO - NIAIST, JAPAN | |
Russell, Robert | |
Yeum, Kyung-Jin |
Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only Publication Acceptance Date: 2/11/2008 Publication Date: 7/7/2008 Citation: Yoshida, Y., Li, L., Duker, J., Niki, E., Russell, R., Yeum, K. 2008. Effect of antioxidants on the oxidative stress in cataract patients. Journal of Federation of American Societies for Experimental Biology. Paper No. 11. Interpretive Summary: Technical Abstract: To determine the relationship between oxidative stress and eye disease, and the impact of antioxidant supplementation, forty subjects (50-70 y, F25, M15) were enrolled in a double blinded randomized study. Subjects were randomized to receive either 1) lutein (12 mg) or 2) lutein (12 mg) + green tea extract (200 mg) for 16 wks, and underwent eye examinations before (wk 0) and after supplementation (wk 16). Lutein is present in human lens and macula. Blood samples were collected at 0, 4, 8, 12 & 16 wks and analyzed for antioxidants by HPLC systems, total antioxidant performance by fluorometry, malondialdehyde by an HPLC with a fluorescent detector, 8-iso-prostaglandin-F2a and total hydroxyoctadecadienoic acid (tHODE) by a GC-MS. Twenty seven subjects (F17, M10) were determined to have cataract at wk 0. In these subjects, tHODE level was significantly higher than the healthy subjects (P<0.05) at wk 0. Lutein supplementation resulted in a significant increase in plasma lutein in cataract patients as early as wk 4 & throughout the study period without any significant change in other antioxidants or oxidative stress biomarkers. Our data suggest that cataract patients were under high oxidative stress and their plasma lutein concentration can be significantly increased by a daily dose of 12 mg lutein, which did not affect overall antioxidant capacity/oxidative status in plasma. |