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ARS Home » Plains Area » Mandan, North Dakota » Northern Great Plains Research Laboratory » Research » Publications at this Location » Publication #214144
Title: Body Condition Score and Day of Lactation Affect Lipogenic mRNA Abundance and Transpription Factors in Adipose Tissue of Beef Cows Fed Supplemental Fat

Author
item MURRIETA, CHARLES - UNIVERSITY OF WYOMING
item LAKE, SCOTT - PURDUE UNIVERSITY
item Scholljegerdes, Eric
item HESS, BRET - UNIVERSITY OF WYOMING
item RULE, DAN - UNIVERSITY OF WYOMING

Submitted to: American Society of Animal Science Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 2/14/2007
Publication Date: 7/1/2007
Citation: Murrieta, C.M., Lake, S.L., Scholljegerdes, E.J., Hess, B.W., Rule, D.C. 2007. Body Condition Score and Day of Lactation Affect Lipogenic mRNA Abundance and Transpription Factors in Adipose Tissue of Beef Cows Fed Supplemental Fat. Jr. of Animal Science 85(Suppl.1):121.

Interpretive Summary:

Technical Abstract: We hypothesized that BCS at parturition and postpartum dietary fat supplementation will alter transcription factors and mRNA abundance of adipose tissue lipogenic and lipolytic enzymes during lactation in beef cows. Our objective was to determine abundance of mRNA for acetyl-CoA carboxylase (ACC), hormone-sensitive lipase (HSL), and lipoprotein lipase (LPL), and transcription factor levels in adipose tissue of 3-yr old Angus × Gelbvieh beef cows nutritionally managed to achieve a BCS of 4 ± 0.07 (BW = 479 ± 36 kg; n = 18) or 6 ± 0.07 (BW = 579 ±53 kg; n = 18) at parturition. Beginning 3 d postpartum, cows within each BCS were assigned to isonitrogenous and isocaloric diets of hay plus low-fat control supplement, or supplements (5% of DMI as fat) with either cracked high-linoleate or cracked high-oleate saf'ower seeds until d 60 of lactation. At d 30 and d 60 of lactation, s.c. adipose tissue biopsies were collected for RNA extraction, quantitative RT-PCR determination of transcript abundance, and Western blot analysis for STAT-5 and PPAR-'. Adipose tissue of BCS 4 cows had less mRNA for LPL (P = 0.001) and HSL (P = 0.09) compared with BCS 6 cows. Abundance of LPL mRNA was lower (P = 0.002) at d 30 postpartum compared with d 60; whereas, HSL mRNA was greater at d 30 (P = 0.001). Cow BCS did not affect (P = 0.35) ACC mRNA; however, it tended to be higher (P = 0.13) at d 60 compared to d 30 of lactation. Abundance of PPAR-' tended (P = 0.13) to be lower in adipose tissue of BCS 4 cows compared with BCS 6 cows. Both STAT-5 (P = 0.0001) and PPAR-' (P = 0.05) were higher at d 30 compared to d 60 postpartum. We conclude that abundance of adipose tissue mRNA for LPL and HSL are influenced by cow BCS, and changes in mRNA abundance during lactation indicates a shift in nutrient partitioning away from the mammary gland to s.c. adipose tissue. Furthermore, STAT-5 and PPAR-' likely play a role in the transcription regulation of LPL and HSL in adipose tissue during lactation in beef cows.