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Title: A NOVEL APPROACH FOR ANALYSIS OF POPLAR WOOD USING TWO-DIMENSIONAL NMR SPECTROSCOPY ON DISSOLVED WHOLE CELL WALLS IN COMBINATION WITH CHEMOMETRICS

Author
item HEDENSTROM, MATTIAS - UMEA UNIV, SWEDEN
item WIKLUND, SUSANNE - UMEA UNIV, SWEDEN
item Ralph, John
item EDLUND, ULF - UMEA UNIV, SWEDEN
item SUNDBERG, BJORN - UMEA UNIV, SWEDEN

Submitted to: Physiologia Plantarum
Publication Type: Proceedings
Publication Acceptance Date: 4/1/2007
Publication Date: 8/12/2007
Citation: Hedenstrom, M., Wiklund, S., Ralph, J., Edlund, U., Sundberg, B. 2007. A novel approach for analysis of poplar wood using two-dimensional NMR spectroscopy on dissolved whole cell walls in combination with chemometrics. Physiologia Plantarum. 130(4):Abstract No. 22.

Interpretive Summary:

Technical Abstract: An understanding of secondary xylem (wood) biosynthesis and the genes regulating this highly complex machinery have many potential applications, and are the central goals within FuncFiber (a Swedish center of excellence in wood science, (www.funcfiber.se). Research on cell wall biosynthesis requires reliable and informative analytical methods for chemical analysis of the cell wall in different genotypes and mutants. Moreover, modifications of the cell wall biosynthesis may yield unexpected secondary effects on wall chemistry and therefore, it is important to utilize analytical techniques that give as complete a picture of the cell wall components as possible. Nuclear Magnetic Resonance (NMR) spectroscopy is a robust and information-rich analytical technique that can be used to detect and characterize the chemical structure of the different cell wall polymers. A method for complete dissolution of the entire secondary cell wall has been developed by Lu et al. (Plant J., 2003); a method that enables the use of two-dimensional NMR experiments in which both the lignin and polysaccharides can be characterized simultaneously. Moreover, recent technical advances have drastically increased the sensitivity of NMR spectrometers. As a result, large numbers of samples can now be analyzed by two-dimensional NMR spectroscopy which allows statistical analysis of the resulting complex spectral data. We have employed chemometric methods to model the correlation between the spectral data and a certain response, e.g., genotype, thereby pinpointing chemical differences in the cell wall induced by the different genetic modifications. Through the use of Partial Least Squares Projections to Latent Structures (PLS), these induced changes can be separated from the inherent biological variation. This combination of two-dimensional NMR spectroscopy and chemometric analysis of wood samples constitutes a novel approach in wood chemistry analysis. We will show some examples that demonstrate that this method can provide information about wood chemistry in transgenic poplar, not possible to obtain by traditional data analysis.