Author
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Wesley, Irene |
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Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 3/11/2007 Publication Date: 3/11/2007 Citation: Wesley, I.V. 2007. Prevalence of Campylobacter and Salmonella in the turkey brooder house [abstract]. 58th North Central Avian Disease Conference Proceedings. p. 19. Interpretive Summary: Technical Abstract: We have previously surveyed market weight turkeys for the effect of transport to and holding at the abattoir for the prevalence of Campylobacter and Salmonella. Whereas Campylobacter is frequently found in the ceca of adult birds (~60%), the prevalence of Salmonella varies considerably from farm to farm. The purpose of this study was to evaluate the point of entry of these bacterial food-borne pathogens into the brooder house, the first stage of commercial turkey production. Two studies were conducted. In summer 2005, birds (100 per time point), were collected on-farm (0, 9 and 16 days of age), transported to the laboratory, euthanized, necropsied, and intestines cultured for Campylobacter and Salmonella. Whereas Campylobacter spp. were not detected in day of hatch poults, Salmonella was isolated from the ceca (3.9%) and yolk sac (0.1%). By day 9, whereas Campylobacter was not detected, Salmonella was frequently isolated from the ceca (55%) and small intestine (45%). By day 16, the estimated prevalence of Salmonella in the ceca (21%) and small intestine (5.1%) had declined. In contrast, Campylobacter was infrequently isolated from either the ceca (2%) or small intestine (3%). In summer 2006, birds 5- to 33- days old were monitored on a second farm under the same ownership. This provided the opportunity to compare conventional culture with two commercially available real-time PCR detection formats. Whereas Campylobacter was not detected in the ceca on 5-day old birds it could be detected on days 20 (92%) and 33 (90%). Salmonella was isolated from nearly all of the 5-day (98%) and 33-day old (96%) birds, at which time the turkeys were moved to the finisher house. At slaughter, Salmonella (4.5%) and Campylobacter (92%) were isolated from the ceca of the market weight (138-day old) birds originating from this flock. Overall, for Salmonella, conventional culture was more sensitive than either of the real-time platforms. For Campylobacter, the two real-time formats differed significantly in their sensitivity due to the number of “error runs” on one of the platforms. We have previously observed high levels of ciprofloxacin resistance in Campylobacter in market weight birds. Antimicrobial profiles of the archived Campylobacter isolates indicated resistance to ciprofloxacin was already present in the young bird. |
