Skip to main content
ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Infectious Bacterial Diseases Research » Research » Publications at this Location » Publication #207317
Title: JDIP Genomics, Antibodies, and Proteomics Core Update

Author
item Paustian, Michael

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 1/18/2007
Publication Date: 1/18/2007
Citation: Paustian, M. 2007. JDIP Genomics, Antibodies, and Proteomics Core Update [abstract]. Johne's Disease Integrated Program Conference.

Interpretive Summary: This work summarizes the activities of the Johne’s Disease Integrated Program (JDIP) genomics, antibodies, and proteomics (GAP) core. The GAP core is a virtual core facility comprised of researchers from several academic and government institutions. Through the GAP core, reagents have been developed and made available to other JDIP researchers. These reagents include bacterial mutants, microarrays, monoclonal antibodies, and purified proteins. This work will be of primary interest to other researchers who have and will continue to utilize the resources of the GAP core in their own studies.

Technical Abstract: The JDIP Genomics, Proteomics, and Antibodies Core has developed several resources that are available for use by JDIP researchers. Five tasks have been completed or are in progress: Task 1 – Transposon mutants: Nearly 24,000 gene disruption M. paratuberculosis mutants are now available for JDIP researchers. 5,000 of these mutants have been or will be sequence characterized, while the remaining mutants are available for screening. A signature tagged mutant library is also available. Task 2 – Microarray: The Core has completed the development and construction of a 70mer oligonucleotide microarray containing probes for all of the predicted coding sequences in the M. paratuberculosis K-10 genome as well as sequences from the M. avium 104 genome. The microarray is available to JDIP researchers and has been used for transcriptional analyses and comparative genomics. Task 3 – ORF clone set: Over 240 M. paratuberculosis fusion proteins are currently available to JDIP researchers. Approximately 118 additional genes will be cloned and made available in the future. Task 4 – Antibodies: A monoclonal antibody (mAb) for CD69, an activation molecule that is upregulated within 4 hours following antigenic stimulation, is available. Additionally, mAbs against a M. paratuberculosis surface protein (MMP - MAP2121c), isocitrate lyase (MAP1643), and dnaK (MAP3840) have been developed. A set of ten strongly reacting mAbs to M. paratuberculosis proteins whose identity is currently unknown have also been developed. The development of a M. paratuberculosis-specific mAb as well as additional mAbs against bovine T regulatory cell markers is ongoing. Task 5 – Services: This activity has been refocused toward providing a web resource for functional genomics tools and data. This web portal will allow JDIP researchers to share data as well as provide access to the tools and expertise available through the GAP Core. As the Core completes its transition to self-sufficiency, the web portal will serve to coordinate the distribution of reagents and data.