Skip to main content
ARS Home » Midwest Area » West Lafayette, Indiana » Crop Production and Pest Control Research » Research » Publications at this Location » Publication #203659
Title: The susceptibility response to BYDV in Avena sativa: Using the wheat gene microarry as a tool for measuring gene expression in oat in response to BYDV infection

Author
item Anderson, Joseph

Submitted to: American Oat Workers Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 7/24/2006
Publication Date: 10/13/2006
Citation: Anderson, J.M. 2006. The susceptibility response to BYDV in Avena sativa: Using the wheat gene microarry as a tool for measuring gene expression in oat in response to BYDV infection. American Oat Workers Conference Proceedings. p. 65.

Interpretive Summary:

Technical Abstract: The development of wheat and barley microarrays for gene expression analyses have opened the ability to identify genes whose expression patterns change relative to some applied treatment. The development of these gene arrays has only been possible because of the large amount of EST sequencing. Because of very limited resources a relatively low level of sequencing has been done in oat. In this study, the wheat microarray was examined as a potential tool for examining global gene expression in oat. The wheat microarray was chosen because it has approximately 55,000 genes arrayed compared to the 23,000 gene barley array. Anecdotal evidence suggests that oat is inherently more susceptible to BYDV than other cereals such as wheat. My laboratory has preliminary data that suggests that Clintland 64, a variety that is highly susceptible to BYDV-PAV, accumulates more of this virus than comparable susceptible wheat lines and some defense response genes are down-regulated after infection. The aim of this project is to begin examining the susceptible response to infection with BYDV-PAV. RNA isolated from Clintland 64 plants that were untreated, infested with nonviruliferous aphids and infested with aphids viruliferous with BYDV-PAV at ten times points post-infestation were initially examined for virus accumulation. Samples before, during and after maximum virus accumulation were used to hybridize to Affymetrix wheat microarrays. The data from these experiments will determine which percentage of the wheat array will be useful in examining oat gene expression and how the plant is responding to virus infection.