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Title: Apoplastic phenol co-oxidation and a novel oxidative burst

Author
item Baker, Con
item AVERYANOV, ANDREY - RES INST PHYTOPATH RUSSIA

Submitted to: Free Radicals in Biology and Medicine
Publication Type: Abstract Only
Publication Acceptance Date: 9/15/2006
Publication Date: 10/15/2006
Citation: Baker, C.J., Averyanov, A. 2006. Apoplastic phenol co-oxidation and a novel oxidative burst. Free Radicals in Biology and Medicine. vol. 41:S58.

Interpretive Summary:

Technical Abstract: The plant apoplast is an important mediator of communication between the cell cytoplasm and its surroundings. Plant cell suspensions offer a convenient model system to gain insight into apoplastic physiology. Here we describe a novel phenomenon that took place when two naturally occurring phenolics were added together to either soybean or tobacco cell suspensions. Acetosyringone and/or hydroxyacetophenone, phenolics found in the extracellular/apoplast of tobacco cells, were added to soybean or tobacco cell suspensions undergoing an oxidative burst. Individually, acetosyringone appeared to be utilized as a typical peroxidase substrate to scavenge hydrogen peroxide, while hydroxyacetophenone was utilized at a much lower rate. However, when added together the rate of utilization of both phenolics increased and surprisingly resulted in the production of hydrogen peroxide. We have further characterized this novel phenomenon in suspension cells. This study demonstrates that certain phenolics in plants can cause co-oxidation which, as in animals, could alter the structure and bioactivity of surrounding phenolics.