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Title: Near-infrared transmission and reflectance spectroscopy for the measurement of dietary fiber in barley

Author
item Kays, Sandra
item SHIMIZU, NAOTO - NFRI, IAA, TSUKUBA, JAPAN
item Barton Ii, Franklin
item OHTSUBO, KEN'ICHI - NFRI, IAA, TSUKUBA, JAPAN

Submitted to: United States-Japan Cooperative Program in Natural Resources
Publication Type: Abstract Only
Publication Acceptance Date: 9/5/2006
Publication Date: 10/21/2006
Citation: Kays, S.E., Shimizu, N., Barton II, F.E., Ohtsubo, K. 2006. Near-infrared transmission and reflectance spectroscopy for the measurement of dietary fiber in barley. United States-Japan Cooperative Program in Natural Resources. P. CE13.

Interpretive Summary:

Technical Abstract: Near-infrared (NIR) transmission and reflectance spectroscopy were investigated as rapid screening tools to evaluate the total dietary fiber content of barley (Hordeum vulgare L.) cultivars. A Foss Grainspec Rice Analyzer and an NIR Systems 6500 spectrometer were used to obtain transmission and reflectance spectra, respectively, of polished grains and ground barley. Total dietary fiber was determined by AOAC Method 991.43. Modified PLS models developed for predicting total dietary fiber, using transmission spectra (850-1048 nm) of polished grains, had SECV of 1.04 % (range 5.8-19.7) and multiple coefficient of determination of 0.82 indicating sufficient accuracy for selecting or rejecting high dietary fiber cultivars. NIR reflectance spectroscopy (1104-2494 nm) of ground barley samples resulted in a model with SECV of 0.52 % (range 5.8-19.7) and multiple coefficient of determination of 0.96. The precision, low cost per sample and speed of measurement of the technique will allow making dietary fiber selection decisions for large numbers of progeny in barley breeding programs.