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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #196028
Title: OATS WITH MAIZE CHROMOSOME AND CHROMOSOME SEGMENT ADDITIONS

Author
item WALCH, MATTHEW - UNIVERSITY OF MINNESOTA
item Rines, Howard
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA
item KOWLES, RICHARD - ST. MARY'S UNIV., MN
item STEC, ADRIAN - UNIVERSITY OF MINNESOTA
item KYNAST, RALF - UNIVERSITY OF MINNESOTA
item OKAGAKI, RON - UNIVERSITY OF MINNESOTA
item JACOBS, MORRISON - UNIVERSITY OF MINNESOTA
item GALATOWITSCH, MARK - UNIVERSITY OF MINNESOTA
item SCHMIDT, CHRISTINA - UNIVERSITY OF MINNESOTA
item HUETTL, PAUL - UNIVERSITY OF MINNESOTA

Submitted to: American Oat Workers Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 5/23/2006
Publication Date: 7/23/2006
Citation: Walch, M.D., Rines, H.W., Phillips, R.L., Kowles, R.V., Stec, A.O., Kynast, R.G., Okagaki, R.J., Jacobs, M., Galatowitsch, M.W., Schmidt, C.M., Huettl, P.A. 2006. Oats with maize chromosome and chromosome segment additions [abstract]. In: 2006 American Oat Workers' Conference Program Book. 2006 American Oat Workers Conference, July 23-26, 2006, Fargo, North Dakota. p. 59. Available: http://wheat.pw.usda.gov/ggpages/oatnewsletter/v50/AOWC/Oat_Book5.pdf.

Interpretive Summary:

Technical Abstract: Oat (Avena sativa L., 2n = 6x = 42) plants crossed with maize (Zea mays L., 2n = 2x = 20) yields both haploid oat plants and plants with one or more maize chromosomes added to a haploid oat genome. Recovery of plants requires embryo rescue following partial or full elimination of the maize chromosomes during embryo development. Doubled haploid plants may be produced either as a result of unreduced gamete formation or through colchicine doubling. Self-fertile single maize chromosome additions to oat (2n = 42 + 2) have been recovered for each of the ten maize chromosomes. Many of these exhibit novel phenotypes resulting from expression of maize genes present. Enzymes associated with C4 photosynthesis have been shown present in addition lines with maize chromosomes carrying genes for these enzymes, phosphoenolpyruvate carboxylase (PEPC) in a maize chromosome 9 addition and pyruvate orthophosphate kinase (PPDK) in a chromosome 6 addition. Plants with both maize chromosomes 6 and 9 present are being made to test possible effects on CO2 compensation points. No instances of enhanced resistance to oat crown rust were found in seedling tests of the set of addition lines. Expression and possible effects of maize systemic resistance genes currently are being studied in these materials. Treatment of these addition lines with gamma irradiation has allowed recovery of "radiation hybrids" with only segments of individual maize chromosome present. The maize chromosome and chromosome segment additions to oat have been distributed to more than 40 labs as valuable tools for physical mapping of maize genes and for other maize genomic studies, as well as being a possible new source of genes for oat improvement.