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Title: OAT-MAIZE ADDITION AND RADIATION HYBRID LINES FOR THE PHYSICAL AND GENETIC MAPPING OF THE MAIZE GENOME

Author
item OKAGAKI, RON - UNIVERSITY OF MINNESOTA
item KYNAST, RALF - UNIVERSITY OF MINNESOTA
item STEC, ADRIAN - UNIVERSITY OF MINNESOTA
item SCHMIDT, CHRISTINA - UNIVERSITY OF MINNESOTA
item JACOBS, MORRISON - UNIVERSITY OF MINNESOTA
item ODLAND, WADE - UNIVERSITY OF MINNESOTA
item CABRAL, CANDIDA - UNIVERSITY OF MINNESOTA
item WALCH, MATT - UNIVERSITY OF MINNESOTA
item GALATOWITSCH, MARK - UNIVERSITY OF MINNESOTA
item HUETTL, PAUL - UNIVERSITY OF MINNESOTA
item SURESH, JAYANTI - UNIVERSITY OF MINNESOTA
item RETZEL, ERNEST - UNIVERSITY OF MINNESOTA
item Rines, Howard
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA

Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 2/19/2006
Publication Date: 3/9/2006
Citation: Okagaki, R., Kynast, R., Stec, A., Schmidt, C., Jacobs, M., Odland, W., Cabral, C., Walch, M., Galatowitsch, M., Huettl, P., Suresh, J., Retzel, E., Rines, H.W., Phillips, R. 2006. Oat-maize addition and radiation hybrid lines for the physical and genetic mapping of the maize genome [abstract]. 48th Maize Genetics Conference Abstracts. p. 110.

Interpretive Summary:

Technical Abstract: Oat-Maize Addition (OMA) lines are available with maize chromosomes 1-10, and ones also with a maize B chromosome, added individually to the oat genome by wide crossing. Previously, maize chromosomes 1-10 from Seneca 60 had been recovered individually in OMA lines. Self-fertile lines transmitting the added maize chromosome were obtained for maize chromosomes 1-9 but only for the short arm of chromosome 10. DNA is now available for OMAs of all 10 maize chromosomes in either a B73 or Mo17 background. Disomic additions have been recovered for chromosomes 1, 4, and 5 of B73 and chromosomes 2, 5, and 10 of Mo17. Gamma irradiation of the OMA lines has yielded several hundred Radiation Hybrid (RH) lines with only a fragment of the maize chromosome, either after partial deletion of the maize chromosome or from translocation of the maize chromosome to an oat chromosome. RH mapping panels are now available for low- and medium-resolution physical mapping, as well as for contig assembly and map-based cloning. The total number of RH lines recovered follows: chromosome 1 (68), 2 (81), 3 (39), 4 (71), 5 (41), 6 (121), 7 (13), 8 (0), 9 (202), and 10 (2). Preferential recovery of RH lines with breaks in the same intervals has been observed in some RH lines. Here, we report on the current status and availabilty of OMA lines and RH mapping panels. This material is based upon work supported by the National Science Foundation under Award No. 0110134.