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United States Department of Agriculture

Agricultural Research Service

Title: Identification of the Genes Associated with Tomato Irregular Ripening Using Microarray Technology

Authors
item McKenzie, Cindy
item Sinisterra, Xiomara - UNIV. OF FLORIDA
item Powell, Charles - UNIV. OF FLORIDA
item Bausher, Michael
item Albano, Joseph
item Shatters, Robert

Submitted to: Entomology International Congress
Publication Type: Abstract Only
Publication Acceptance Date: July 9, 2004
Publication Date: August 15, 2004
Citation: Mckenzie, C.L., Sinisterra, X.H., Powell, C.A., Bausher, M.G., Albano, J.P., Shatters, R.G. 2004. Identification of the genes associated with tomato irregular ripening using microarray technology. Entomology International Congress.

Technical Abstract: Tomato irregular ripening (TIR) disorder has been associated with silverleaf whitefly (SLW) (Bemisia argentifolli Bellows and Perring) feeding. Symptoms of this physiological disorder do not appear on tomato foliage where whitefly feed, but appear as an uneven fruit color development. Plants infested with SLW can develop fruit with external longitudinal white or yellow streaks resulting in unmarketable fruit. TIR internal symptoms consist of a lack of internal coloring of the fruit, thereby reducing post harvest quality. The mechanism(s) associated with the development of TIR has not been identified. Gene array technology was applied to TIR as a model system. Our objective was to study gene expression over time in tomato plants subjected to a moderate infestation of whitefly and resulting in the development of TIR. Multiple plant harvests of old and young leaves, stems, roots, flowers and fruit from tomato with and without whitefly were processed for nutritional analysis and RNA extraction RNA was labeled and hibridized to the gene array membrane to determine which genes SLW feeding influences. Plants infested with whitefly were larger, produced more leaves, flowers and fruit, but significantly less fruit ripened compared to plants without whitefly. Plant nutrition and RNA profiling results will be presented.

Last Modified: 11/28/2014