Author
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MA, X - UNIV OF MISSOURI |
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Gustafson, J |
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Submitted to: University of Missouri Life Sciences Week
Publication Type: Abstract Only Publication Acceptance Date: 3/7/2003 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: The coding sequences of triticale were studied by using RFLP and AFLP analyses with fifty oat EST clones and forty primer pairs, respectively, to analyze the genome banding profiles of four primary triticales and their wheat and rye progenitors. The results showed that almost 100 percent of the EST bands that appeared in wheat also appeared in triticale, whereas about 60 percent of the EST bands that appeared in rye were present in triticale. The results also indicated that the majority of AFLP bands present in the wheat parents were maintained in the triticales. Compared to the methylation-unsensitive primers EcoRI/MseI (E/M), the AFLP bands amplified by methylation-sensitive primers PstI/MseI (P/M) showed much higher conservation of wheat parental bands in all the triticales studied. Furthermore, for both E/M and P/M primers, the hexaploid wheat genomes were more conserved in triticale as compared to tetraploid wheat genomes. However, for both sets of primers and for both hexaploid and octoploid triticales, the rye genome underwent great changes when placed in triticale. More than 70 percent of the rye parental AFLP bands were absent in triticale. The overall results suggested that the unmenthylated low-copy-coding sequences present in wheat tended to be highly conserved in triticale whereas a majority of the rye genome was involved in a very high level of variation regardless of low-copy-coding sequences or non-coding sequences and presence in a hexaploid or octoploid triticale. |
