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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Publications at this Location » Publication #146222
Title: GENOME STUDIES OF THE WHEAT/RYE HYBRID TRITICALE

Author
item MA, X - UNIV OF MISSOURI
item RODRIGUEZ-MILLA, M - UNIV OF MISSOURI
item Gustafson, J

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/10/2003
Publication Date: 1/12/2003
Citation: MA, X.F., RODRIGUEZ-MILLA, M.A., GUSTAFSON, J.P. GENOME STUDIES OF THE WHEAT/RYE HYBRID TRITICALE [abstract]. PLANT AND ANIMAL GENOME ABSTRACTS. 2003. p. 165.

Interpretive Summary:

Technical Abstract: The genome variations of triticale were studied by using 28 AFLP primer pairs, which included both EcoRI/MseI (E/M) and PstI/MseI (P/M) primer combinations, to analyze the genome banding profiles of four primary triticales and their wheat and rye progenitors. The results indicated that the majority of AFLP bands present in the wheat parents were maintained in the triticales. Compared to E/M primers, the AFLP bands amplified by P/M primers showed much higher conservation of wheat parental bands in all the triticales studied. Furthermore, for both E/M and P/M primers, the hexaploid wheat genomes were more conserved in triticale as compared to tetraploid wheat genomes. However, for both sets of primers and for both hexaploid and octoploid triticales, the rye genome underwent great changes in triticale. More than 70% of the parental bands present in rye were absent in triticale. The results suggested that the unmethylated low-copy sequences present in wheat tended to be highly conserved in triticale whereas a majority of the rye genome was involved in a very high level of variation regardless of low-copy or repetitive sequences and presence in a hexaploid or octoploid triticale. The results also showed that when a parental AFLP band was present in both wheat and rye, the tendency of that AFLP band to be present in the triticale was much higher than if it was present in only one of the progenitors, especially in octoploid triticales and/or in P/M primers.