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Title: MEASUREMENT OF TOTAL DIETARY FIBER IN MILLED BARLEY USING NEAR-INFRARED TRANSMITTANCE SPECTROSCOPY

Author
item SHIMIZU, NAOTO - NATL FOOD RES INST, JAPAN
item Kays, Sandra
item Barton Ii, Franklin
item FUJITA, MASAYA - NATL FOOD RES INST, JAPAN
item ISHIKAWA, NAOYUKI - NATL FOOD RES INST, JAPAN
item ODA, SYUNSUKE - NATL FOOD RES INST, JAPAN
item KIMURA, TOSHINORI - NATL FOOD RES INST, JAPAN
item OHTSUBO, KEN'ICHI - NATL FOOD RES INST, JAPAN

Submitted to: Nippon Shokuhin Kagaku Kogaku Kaishi
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/29/2002
Publication Date: 11/1/2002
Citation: SHIMIZU, N., KAYS, S.E., BARTON II, F.E., FUJITA, M., ISHIKAWA, N., ODA, S., KIMURA, T., OHTSUBO, K. MEASUREMENT OF TOTAL DIETARY FIBER IN MILLED BARLEY USING NEAR-INFRARED TRANSMITTANCE SPECTROSCOPY. NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI. 2002. V. 49(9). P. 620-623.

Interpretive Summary: Barley is grown for two purposes, its use in cereals and malting for beverages. The principle difference between to two types of barley is its dietary fiber content. This assay is complicated, arduous and expensive. Near Infra-red spectroscopy has been used to measure dietary fiber in barley in a collaborative project between the Quality Assessment Research Unit in Athens, GA and the National Food Research Institute in Tsukuba, Japan. The results of this study can be used for screening barley breeding programs in the U.S. and Japan. This study is a tangible product of the U.S. Japan Natural Resource Panels (UJNR) Protein Panel.

Technical Abstract: Whole-grain milled barley (n=56) was scanned using a near-infrared transmittance spectroscopy (NIT)(850-1048nm) and total dietary fiber (TDF) was determined for each cultivar by AOAC enzymatic-gravimetric method (Method 991.43). The validation statistics of PLS models using calibration (n=28) and validation (n=28) sets were the standard error of performance (SEP) of 0.89~1.03, the coefficient of determination (R**2) of 0.83~0.88, Bias of -0.25~0.17 and the ratio of the SEP to the standard deviation of the original data (RPD) of 2.5~2.9. This method could be applied to rough screening in barley breeding programs.