Skip to main content
ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Publications at this Location » Publication #140809
Title: COMPLETE CDNA CLONING AND POLYMORPHISMS AT PORCINE BPI: ASSOCIATIONS WITH BACTERIAL LOAD AND IMMUNE RESPONSE TRAITS IN PIGS

Author
item SHI, XIAN-WEI - IOWA STATE UNIVERSITY
item MELLENCAMP, M - SYGEN INTERNATIONAL
item Stabel, Thomas
item GALINA-PANTOJA, L - SYGEN INTERNATIONAL
item BASTIAANSEN, J - SYGEN INTERNATIONAL
item TUGGLE, C - IOWA STATE UNIVERSITY

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 1/15/2003
Publication Date: 1/15/2003
Citation: SHI, X., MELLENCAMP, M.A., STABEL, T.J., GALINA-PANTOJA, L., BASTIAANSEN, J., TUGGLE, C.K. COMPLETE CDNA CLONING AND POLYMORPHISMS AT PORCINE BPI: ASSOCIATIONS WITH BACTERIAL LOAD AND IMMUNE RESPONSE TRAITS IN PIGS. PLANT AND ANIMAL GENOME CONFERENCE. 2003. ABSTRACT P. 231.

Interpretive Summary:

Technical Abstract: The bactericidal permeability-increasing (BPI) gene encodes a neutrophil protein with inhibitory/killing functions against gram-negative bacteria. We investigated BPI as a candidate gene for resistance to Salmonella cholerasuis (SC) in pigs. We cloned and sequenced a full-length BPI cDNA and identified polymorphisms at BPI; a SNP in intron 4, a 300-bp indel in intron 10, and two missense mutations in complete linkage disequilibrium within exon 10 (A354T-R384L). The effect these polymorphisms may have on resistance to infection was tested in two experiments. Study 1: 126 piglets were intranasally challenged with 10**9 cfu SC. Blood was collected before and six days post-challenge for complete blood count. Feces were collected daily and quantitatively cultured for SC. All animals were genotyped for BPI polymorphisms. Statistical analysis showed all three marker systems assigned animals to classes which showed significant differences (P values ranged from .006 to .03) in day 6 fecal shedding. Neutrophil number and change in lymphocyte number were also associated with BPI genotypes. Study 2: 216 F2 piglets from a SC resource family were orally challenged with 8 x 10**8 cfu SC. Many innate immunity traits and post-challenge spleen and liver bacterial counts were collected. The BPI SNP and missense polymorphisms tested across this population were significantly associated with neutrophil bacteria uptake, and the BPI missense polymorphisms were associated with bacterial counts in liver (P < .1) and on lymphocyte ConA stimulation response post-challenge (P < .0001). These results suggest that BPI variation may control, in part, response to Salmonella infection in pigs.