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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Livestock Bio-Systems » Research » Publications at this Location » Publication #136945
Title: THE EFFECT OF PORCINE OVIDUCTAL FLUID ON IN VITRO DEVELOPMENT TO BLASTOCYST STAGE BY HOMOLOGOUS AND HETEROLOGOUS EMBRYOS

Author
item STELL, SLYVIA - MEHARRY MED COLLEGE, TN
item Ford, Johny
item NIAZ, MOHAMMAD - MEHARRY MED COLLEGE, TN
item DIEHL, JOHN - CLEMSON UNIVERSITY, SC
item ARCHIBONG, ANTHONY - MEHARRY MED COLLEGE, TN

Submitted to: Biology of Reproduction Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2002
Publication Date: 7/1/2002
Citation: Stell, S., Ford, J.J., Niaz, M.S., Diehl, J.R., Archibong, A.E. 2002. The effect of porcine oviductal fluid on in vitro development to blastocyst stage by homologous and heterologous embryos [abstract]. Biology of Reproduction Abstracts. 66 (Supplement 1):151.

Interpretive Summary:

Technical Abstract: In vitro fertilization rates for aspirated human oocytes are approximately 80 - 90%; however, implantation rates among these embryos are low, probably due to asynchronous transfers of cleavage stage embryos into the uterus. Improvement of in vitro culture conditions for the production of morulae/blastocysts could improve pregnancy rates post embryo transfer (synchronous transfer). As a prelude to testing the embryotrophic efficacy of porcine oviductal fluid (POVF) on higher mammalian embryos (primates included), we studied the embryotrophic effects of POVF on mouse embryo during in vitro cultures. Fluids from the oviducts of cycling sows were harvested via surgically implanted cannulae from pro-estrus to early luteal phase of the estrous cycle and volume determined. RIA was used to determine E2 and P4 contents of collected POVFs. POVF collected at the first observed rise in P4 was used for embryo cultures. In expt. 1, porcine zygotes were cultured in modified Kreb's Ringer buffer (control; KRB) or KRB + POVF (final concentrations = 10%). In expt. 2, 2-cell mouse embryos were cultured in Whitten's medium supplemented with CZBt medium containing growth factors (WM-CZBt; control) or WM-CZBt-POVF containing 10% POVF. All embryos were cultured for 5 days. Data on the POVF volume and the concentrations of E2 and P4 were analyzed by split plot analysis of variance and the differences among means determined by orthogonal contrasts. Data on percentage blastocyst formation were compared by Chi-square. E2 concentrations remained maximal during pro-estrus. On the day of detected estrus, concentrations of E2 declined. P4 concentrations were below the sensitivity of the assay until 48 hours post estrus. As POVF P4 subsequently increased, the volume of POVFs decreased. More pig embryos cultured in KRB + POVF (73%) attained blastocyst stage than those cultured in KRB (27%; p<0.05). Similarly, more mouse embryos cultured in WM-CZBt-POVF (70%) attained blastocyst stage than those cultured in WM-CZBt (36%; p<0.05). This study indicates that supplementation of in vitro culture media with POVF supported homologous and heterologous embryos development similarly. This supports a non-species specific embryotrophic effect. (Supported by SCORE Grant 2S06GM08037-28)