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Title: GENETIC MAPPING OF MAIZE MUTANTS WITH SSR MARKERS

Author
item CARSON, C - UNIV OF MISSOURI
item ROBERTSON, J - UNIV OF MISSOURI
item LEWIS, N - UNIV OF MISSOURI
item BENNETT, J - UNIV OF MISSOURI
item SPAIN, J - UNIV OF MISSOURI
item CONERLY, P - MISSISSIPPI STATE UNIV
item HAMPTON, R - UNIV OF MISSOURI
item MELIA-HANCOCK, S - UNIV OF MISSOURI
item NEUFFER, G - UNIV OF MISSOURI
item Coe Jr, Edward

Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 3/14/2002
Publication Date: 3/14/2002
Citation: Carson, C., Robertson, J., Lewis, N., Bennett, J., Spain, J., Conerly, P., Hampton, R., Melia-Hancock, S., Neuffer, G., Coe Jr, E.H. 2002. Genetic mapping of maize mutants with ssr markers. Maize Genetics Conference Abstracts. V.44:75.

Interpretive Summary:

Technical Abstract: Mapping of mutants in the Maize Mapping Project seeks to increase the value of the mutant resource with map information. Because the number of mutants is enormous, and is ever growing, we have worked to increase the rate and resolution by which mutants can be mapped with molecular markers. By production of F2 families, large numbers of mutants can be processed each season. Most of the mutants studied to date are derived from diverse and relatively unpredictable backgrounds, so four inbred parents, A619, A632, B73, and Mo17, have been selected for crosses to increase the chances for polymorphism generally, and sometimes predictably. For our purposes, the mutants can be divided into two groups: 1) placed mutants that are known to be located on a particular chromosome or chromosome arm/segment, and 2) unplaced mutants, which account for 75% of more than 4500 mutants known. We further divide the mutants into three categories: kernel, seedling, and adult plant. Map information is obtained using SSR markers detected by PCR and agarose gel electrophoresis. Of 596 placed (largely by B-A translocations) EMS-mutants (Neuffer) and another 234 classic mutant (Maize Genetics Cooperation - Stock Center) F2 family sets (from 1-4 different families per mutant), 343 seedling and adult and 133 kernel mutants have been grown for collection through 2001. Predicted mutants appear in more than 80% of the families. These were analyzed using bulk-segregant analysis (BSA) of pools (homozygous pools vs. segregating pools). As many as 19% were either not on the arm tested, or the homozygotes were too difficult to discern accurately. Of 225 placed mutants confirmed, over 40% have been assessed with individual segregational analysis and greater than 95% were verified. Another 200 placed seedling mutants are currently in process. In addition, we have now established a system for mapping unplaced mutants using BSA. Whole-genome BSA has placed more than 52 unplaced mutants through 2001. Many new mutants are also found and mapped from EMS-mutant families (e.g., numerous new small seedling mutants appear in sandbench cultures). The method also produces false positives, unlinked markers that appear to be linked as a result of chance; although, cases of multiple unlinked gene interactions have also been identified. Funded by NSF Plant Genome Grant DBI 9872655.