Location: Cereal Disease Laboratory
Project Number: 3640-21220-021-27
Start Date: Aug 01, 2014
End Date: Jul 31, 2015
Durum cultivar Kronos was mutated with EMS that generates either single nucleotide mutations and or small deletions in the genome. The DNA from mutants will be isolated and exomes for about 82,000 genes will be captured using standard protocol. These exomes will then be sequenced and compared with that of parental lines to determine the mutations in each specific lines. These data will be uploaded into a database for easy search of mutations in specific genes. Additionally, the seeds of these lines will be made available for screening against known rust or Fusarium isolates. Any change from the Kronos parent reaction to a disease would indicate a mutation in a gene involved in host-pathogen interaction. These genes can then be identified by looking at the mutations in that specific line. Further analysis by both groups will allow identification of candidate genes and characterization of the molecular pathways. Thus we can easily go from a gene sequence to a phenotype and/or from a phenotype to a gene sequence utilizing this amazing resource.