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United States Department of Agriculture

Agricultural Research Service

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Research Project: Characterization and Manipulation of the Prophages/phages of 'candidatus Liberibacter Asiaticus' for the Control of Citrus Huanglongbing

Location: Subtropical Plant Pathology Research

Project Number: 6618-22000-039-50
Project Type: Reimbursable

Start Date: May 01, 2014
End Date: Apr 30, 2017

Objective:
1. Characterize Pr-D (FP3) and its role and disease suppression. 2. Investigate the dynamics of the prophages/phages in Las bacteria by revealing the variations in gene expression and recombination. 3. Identify critical elements, such as heat and chemical stress that facilitates lytic activities of the prophages.

Approach:
This project will be accomplished by: 1) identification and characterization of type D phage/prophage in Las bacteria via purification of phage particles by ultracentrifugation, rolling circle amplification, and cloning; 2) Gene expression analyses for hypothetical protein genes encoded in the prophage regions; 3) Investigation of stress effect (chemical and physical conditions) on the genes involved in the phage lytic cycle; and 4)Improvement of Las culture using Las inoculum with single or incomplete prophage/phage.

Last Modified: 7/30/2014
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