Development of High Throughput Genome and Transcriptome Sequences for Tropical Perennial Crops
Sustainable Perennial Crops
Project Number: 1245-21220-252-25
Specific Cooperative Agreement
Start Date: Jun 26, 2014
End Date: May 25, 2019
To support research through collaborations that provide high throughput next generation sequencing services for tropical and semitropical plants; phytopathogenic fungi and bacteria; plant insect pests and their associated host plants.
The data generated through the collaboration will be used in genomic and transcriptome analysis and genetic diversity analysis. ARS desires the Cooperator to provide goods and services necessary to develop appropriate RNA, DNA, and cDNA libraries from ARS provided materials. The Cooperator will provided sequencing and data processing to ARS in the form of usable sequencing data and well as providing the raw data.
The Cooperator will produce appropriate sequencing libraries for infected and non-infected control material and conduct high throughput sequencing. ARS will provide the Cooperator with isolated DNA and or RNA. The Cooperator will conduct initial alignment and comparison analysis and will send the data to ARS or to a third party designated by ARS.