Start Date: May 17, 2014
End Date: May 16, 2015
Project 1: We will conduct two backcrosses of the transgenic plants into the Rasmusson genetic background and during the introgression process we will select for expression of the transgene. Homozygous lines will be screened for FHB resistance in the field. Obtain genetic stocks segregating for the chromosome 6H bin 7 region and select recombinants from ~5,000 individuals using flanking markers. Screen the recombinants for FHB severity in the field and assess protein content in the mature grain. Project 2: Introgress the HvUGT13248 transgene into two elite cultivars: Linkert and Rollag. Characterize the accumulation of trichothecenes and trichothecene-glucoside conjugates in transgenic wheat carrying HvUGT13248. All genes that exhibit resistance will be transformed into wheat and tested in the greenhouse and field.