Location: Floral and Nursery Plants Research Unit
Project Number: 8020-22000-049-00
Start Date: Apr 29, 2014
End Date: Aug 31, 2017
Apply appropriate molecular markers identified to molecularly identify anastomosis groups (AGs) of Rhizoctonia solani and develop in vitro assays to determine fungicide sensitivity of different AGs affecting turfgrasses, in order to allow selection of appropriate materials for effective disease control.Identify various isolates of Rhizoctonia solani pathogenic to ornamentals, vegetables, and turfgrasses to the levels of anastomosis groups (AGs) and subgroups by developing UP-PCR genome fingerprint-based, sequence-characterized amplified region (SCAR) markers and cross-blot hybridization assays. Using high throughput molecular methods, analyze the genomes, transcriptomes and 2-dimensional proteomes of selected pathogenic AGs of R. solani to catalog genes and develop microsatellite markers, which will aide in pathogen identification and characterization of genes with roles in host specificity, fungal biology, overwintering, virulence, and fungicide resistance. Develop in vitro assays following the "poisoned agar" technique to determine sensitivity of molecularly identified AGs and subgroups of R. solani to commonly used fungicides, in order to allow selection of appropriate fungicides for effective disease control.