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United States Department of Agriculture

Agricultural Research Service

Research Project: Developing Pinto Bean Breeding Lines with Multiple Resistance to Diseases of Importance in North Dakota

Location: Vegetable and Forage Crops Production Research

Project Number: 5354-21220-018-08
Project Type: Reimbursable

Start Date: Jan 01, 2014
End Date: Aug 01, 2015

Objective:
Generate advanced pinto bean breeding lines with multiple resistance to combat the emergent races/strains of pathogens in ND/MN causing anthracnose, common bacterial blight, and bean rust diseases, in combination with good agronomic performance specifically targeted for the ND/MN growing conditions.

Approach:
The three scientists involved in this project will discuss results after each of the steps outlined in the plan below to assess the progress and potential success of the project. Procedures may be adapted if needed based on the information accumulated. • Marker-assisted backcrossing will be used to introduce the most adapted sources of the Co-42, SU91, and Ur-5 resistance genes into elite pinto bean lines and cultivars (eg. Lariat, La Paz, Long’s Peak, Stampede), with high agronomic performance in North Dakota. • The BCnF1 and BCnF2 populations will be assayed for the markers. Those BCnF1 plants with one or more of the markers will be used as parents in subsequent backcrosses and BCnF2 plants with the markers will be selfed to generate F3 lines. The F3 lines will be tested in progeny rows in winter nurseries and selected for general agronomic performance and seed quality in non-disease environments. The selected F3 lines will be bulk harvested as F3:4 lines. • The F3:4 lines will be tested for uniform disease reaction using standard greenhouse and field screening protocols. The F3:4 lines exhibiting disease resistance to one or more of the emergent races/strains of the anthracnose, common bacterial blight, and bean rust pathogens will be entered in preliminary yield trials. • The F3:4 lines that perform well in in the preliminary yield trials will be bulk harvested and retested as F3:5 lines for disease reaction and agronomic performance • Selected lines with high levels of resistance and best agronomic performance will be sent to winter nurseries (New Zealand and/or Puerto Rico) for seed increases, generation advancing, and additional selection. • Simultaneously, screening of NDSU breeding lines will be performed in the greenhouse for the three diseases to identify additional lines that contain either one or a combination of these resistance genes. • Field trials with advanced lines will be performed in North Dakota and Washington to evaluate agronomic performance and make final recommendations.

Last Modified: 9/10/2014
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