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United States Department of Agriculture

Agricultural Research Service

Research Project: Development and Testing of a Transgenic Male-Only Strain of the Screwworm, Cochliomyia Hominivorax (Diptera, Calliphoridae)

Location: Screwworm Research

Project Number: 3094-32000-040-05
Project Type: Interagency Reimbursable Agreement

Start Date: May 01, 2014
End Date: Dec 31, 2014

Objective:
Continue effort to develop a male only strain of screwworm by applying transformation protocols previously developed while also expanding to incorporate approaches successful with Lucilia cuprina.

Approach:
The project has advanced to the stage where evaluations of existing female conditional lethal vectors have been initiated in the Screwworm Research Unit's facility at Pacora, Panama: this work will continue. We will also develop a second vector system as a contingency in the event the current vectors do not meet the Eradication Program's needs. We will: 1. Develop tetracycline repressible female lethal conditional lethal cloning vector based on improved piggybac transposable element/helper system, C. hominivorax transformer sex-specific splicing intron, tTAV coding region, and tetO operon/promoter. 2. Evaluate the new vector through transformation experiments in the secondary screwworm Cochliomyia macellaria before transferring to C. hominivorax (in Panama).

Last Modified: 12/21/2014
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