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United States Department of Agriculture

Agricultural Research Service

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Research Project: Identifying Genetic Pathways Involved in Iron Efficiency of Soybean

Location: Corn Insects and Crop Genetics Research

Project Number: 5030-21220-005-37
Project Type: Reimbursable

Start Date: Apr 09, 2014
End Date: Dec 30, 2014

Objective:
1. Identify the master regulator(s) of iron deficiency response in soybean; 2. Determine if the genetic pathways controlling the iron stress response overlap with other nutrient stress responses; 3. Develop molecular markers for the genes identified in objectives 1 and 2 that can be used for marker assisted selection by breeders to improve soybean agronomic performance

Approach:
1. Use virus induced gene silencing (VIGS) to turn off candidate genes in the iron stress response. If silencing results in improvment or worsening of IDC symptoms use RNA-seq to characterize the response; 2. Test VIGS constructs developed under objective 1 for involvement in other nutrient stress responses (Nitrogen, Phosphate, Calcium, Sodium) using hydroponics; 3. Once genes improving nutrient stress responses are confirmed, we will use sequence differences between genotypes to develop molecular markers that could be broadly used across soybean cultivars.

Last Modified: 12/18/2014
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