Location: Crops Pathology and Genetics Research
Project Number: 5306-21000-022-00
Start Date: Dec 15, 2013
End Date: Dec 14, 2018
Objective 1: Test the strategy of upregulating the key ABA biosynthesis gene, NCED, with a plant-derived stress-inducible promoter pRD29A (cloned from Arabidopsis); use a chemically-induced system (alcohol-inducible promoter, ALC) to up-regulate the expression of NCED in petunia. Objective 2: Biological materials established or collected from Objective 1 will be subjected to either physiological, or biochemical and molecular analysis. Objective 3: Use Four o'clock (Mirabilis jalapa) as a model system for studying the molecular regulation of ethylene insensitive flower senescence. Four o'clock tissues from leaves (young and senescing), stems, roots, whole flowers (no petals) and petals (four stages, before opening, just fully-opened, senescing and wilted) will be harvested.