Location: Vegetable Crops Research Unit
Project Number: 3655-21220-002-13
Start Date: Dec 15, 2013
End Date: Dec 14, 2016
The approach includes a combination of cellular, molecular genetic, and bioinformatic methodologies to elucidate mechanisms involved in the interaction between P. infestans effectors and target host proteins. We will use stable transgenic potato expressing specific P. infestans effectors and RB in pathogen inoculation studies to test whether the presence of some effectors alone is responsible for resistance suppression. We will also use site-directed mutagenesis to identify RB amino acids required for interaction with effectors and abrogation of suppression. Finally, using information from site-directed mutagenesis and/or structure-function analyses, we will use PCR and DNA sequencing to identify RB variants from wild potato species that avoid suppression and, thereby, confer broader spectrum resistance.