Location: Crop Diseases, Pests and Genetics
Project Number: 2034-22000-010-15
Start Date: Jan 01, 2014
End Date: Sep 30, 2016
Electrical penetration graph technology will be used to measure and apply a standardized duration of feeding by nymphal lygus bugs (one at a time) to multiple cotton squares (flower buds). Plants will then be transferred to a greenhouse and held for varying holding periods (e.g., 0, ½, 1, 2, and 4 days). The test will be replicated 3 – 4 times per holding period, for a total of 15 – 20 insects and squares. After the holding periods are completed, fed-upon cotton squares will be excised, fixed, dehydrated, embedded in paraffin, sectioned, mounted on slides, stained, and examined using compound light microscopy. Anatomical changes due to damage and/or healing will be visually correlated with types of waveforms/feeding performed during the standardized duration.