Start Date: Oct 01, 2013
End Date: Aug 31, 2016
Removal of N, P, and other constituents will be optimized in influent and effluent of identical ponds. Algal biomass (harvested by bioflocculation+settling) will be analyzed for N, P, protein, carbohydrates, and profiles of fatty and amino acids. Pathogen, total bacterial and algal communities in raw and feed-processed algal biomass will be analyzed using metagenomics such as 454 pyrosequencing and GeoChip. Potential toxicity of algal biomass will be studied using toxicity evaluation of cell–free extracts on cultured mammalian cells. A TC 20 Cell counter (BioRad Laboratories) will be used to monitor toxicity events on treated cells using trypan blue staining. Cytotoxic positive samples will be tested for both presence and concentration of known cyanobacterial toxins. The researchers have decades’ experience in algae production, wastewater treatment, and food safety.